產品簡介
SequaGel MD 測序SequaGelTM 突變檢測膠
公司簡介
上海復祥生物科技有限公司是一家專業從事原代細胞、細胞系；菌種；及細胞因子相關產品的銷售企業。公司建有自己的細胞庫和菌種庫。細胞庫管理規范，提供的細胞株背景清楚，提供參考文獻和最優培養條件，公司引進ATCC細胞500余種，ATCC菌種800余種。并和國內各大保藏中心有密切聯系，細胞種類齊全，質量保證！是生命科學領域不可缺少的一部分！
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產品說明
SequaGel MD 測序SequaGelTM 突變檢測膠
- Point Mutation Analysis
- SSCP Analysis
- Heteroduplex Analysis
Catalog Number:SG EC-845
1.用于檢測微小突變差異的專業產品.
2.適用于點突變分析.
3.適用于單鏈構象多態性分析（SSCP Analysis）.
4.適用于異源雙鏈分析 （Heteroduplex Analysis）.
得益于相對遷移率的高分辨率，SequaGelTM突變檢測膠允許發現DNA序列中的微小突變差異。SequaGelTM突變檢測膠采用保護的配方，提供2X濃度的貯存液用于單鏈構象多態性和異源雙鏈的分析。異源雙鏈試劑盒由SequaGelTM突變檢測膠溶液和三色上樣緩沖液組成，單鏈構象多態性試劑盒由SequaGelTM突變檢測膠溶液和SSCP終止液組成。100-300bp的SSCP核酸樣品建議使用0.5X濃度的SequaGelTM突變檢測膠，更小的片段則建議使用更高濃度（0.75X）的膠，比較大的片段則可以使用較低濃度（0.4X）的膠。異源雙鏈的核酸樣品在不超過900bp時均推薦使用1X濃度的膠。
SequaGel MD permits minor mutational differences in DNA sequences to be detected as a high resolution relative mobility (Rf) shift. SequaGel MD is a proprietary formulation, supplied as a 2X stock, designed to resolve sequence related differences by SSCP (Single Strand Conformational Polymorphism) and Heteroduplex Analysis.
DNA mutations, or sequence modifications, are readily associated with specific disease states. Since a variation of a single nucleotide in a sequence may indicate a significant genetic anomaly, an extremely sensitive method to analyze these mutations is necessary.
Two principle methods of conformational analysis have been developed, SSCP Analysis and Heteroduplex Analysis. SequaGel MD is available as a monomer solution packaged with Triple Dye Loading Buffer in the SequaGel MD Heteroduplex Kit or with SSCP Stop Solution in the SequaGel MD SSCP Kit.
SequaGel MD is designed to run SSCP samples in the 100-300 nucleotide range at a 0.5X concentration. Small fragments may be better resolved by using higher concentration (0.75X) gels. Larger fragments may be better resolved by running lower concentration (0.4X) gels. SequaGel MD is designed to run heteroduplex analysis on DNA fragments up to 900 bases at a 1X concentration.
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