CRL-2007 JB6 Cl 30-7b 小鼠表皮細胞 ATCC 細胞|細胞系|細胞株|腫瘤細胞|細胞|貼壁細胞|懸浮細胞|,細胞庫管理規(guī)范,提供的細胞株背景清楚,提供參考文獻和Z優(yōu)培養(yǎng)條件,
CRL-2007 JB6 Cl 30-7b 小鼠表皮細胞
|
ATCC® Number:
|
CRL-2007?
|
Price:
|
$429.00
|
|
Designations:
|
JB6 Cl 30-7b
|
|
Depositors:
|
NH Colburn
|
|
Biosafety Level:
|
1
|
|
Shipped:
|
frozen
|
|
Medium & Serum:
|
See Propagation
|
|
Growth Properties:
|
adherent
|
|
Organism:
|
Mus musculus (mouse)
|
|
Morphology:
|
epithelial
|
|
Source:
|
Organ: skin
Strain: BALB/c
Tissue: epidermis
Disease: normal
|
|
Permits/Forms:
|
In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
|
|
|
|
Tumorigenic:
|
No
|
|
Age:
|
newborn
|
|
Comments:
|
This is one of several lines that are useful in studies of the molecular events in tumor promotion and for development of promotion assays (see also ATCC CRL-2002 - RT101, ATCC CRL-2010 - JB6 Cl 41-5a and ATCC CRL-2012 - T36274).
The JB6 Cl 30-7b cell line was derived from primary cultures of neonatal BALB/c epidermal cells that had been treated with dimethylsulfoxide (DMSO, 0.01 to 0.1 %).
The line is resistant to promotion of transformation (P-) by phorbol esters.
These cells can be used in comparisons with P+ cells for studying differential responses to tumor promoters.
Never allow the cells to become confluent.
The cells must be carried at low density to avoid spontaneous transformation.
|
|
Propagation:
|
ATCC complete growth medium: Minimum essential medium (Eagle) with 2 mM L-glutamine, 95%; fetal bovine serum, 5%
Temperature: 37.0°C
|
|
Subculturing:
|
Protocol: The cells must be carried at low density to avoid spontaneous transformation.Volumes used in this protocol are for 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes. 1. Remove and discard culture medium.2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin-0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.3. Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes). Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.4. Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting. 5. Add appropriate aliquots of the cell suspension to new culture vessels. An inoculation density of 2 x 104 viable cells per 25 sq. cm. flask is recommended or a spilt ratio of 1:6 to 1:8.6. Incubate cultures at 37°C.
Subcultivation Ratio: An inoculation density of 2 X 10 exp4 viable cells per 25 sq. cm. flask is recommended
Medium Renewal: 1 to 2 times per week
Rap the flask smartly against a hard surface to get a single cell suspension.
Add fresh medium, aspirate and dispense into new flasks. Culture at 36C in a humidified atmosphere of 5% CO2 - 95% air.
|
|
References:
|
22309: Colburn NH, et al. A cell culture assay for tumor-promoter-dependent progression toward neoplastic phenotype: detection of tumor promoters and promotion inhibitors. Teratog. Carcinog. Mutagen. 1: 87-96, 1980. PubMed: 6119803
22427: Colburn NH, et al. Correlation of anchorage-independent growth with tumorigenicity of chemically transformed mouse epidermal cells. Cancer Res. 38: 624-634, 1978. PubMed: 626967
22908: Colburn NH, et al. Tumour promoter induces anchorage independence irreversibly. Nature 281: 589-591, 1979. PubMed: 492322
22959: Bernstein LR, Colburn NH. AP1/jun function is differentially induced in promotion-sensitive and resistant JB6 cells. Science 244: 566-569, 1989. PubMed: 2541502
23279: Colburn NH, et al. Dissociation of mitogenesis and late-stage promotion of tumor cell phenotype by phorbol esters: mitogen-resistant variants are sensitive to promotion. Proc. Natl. Acad. Sci. USA 78: 6912-6916, 1981. PubMed: 6947266
|
|
上海復祥生物提供 ATCC 細胞|細胞系|細胞株|腫瘤細胞|細胞|貼壁細胞|懸浮細胞|,細胞庫管理規(guī)范,提供的細胞株背景清楚,提供參考文獻和優(yōu)培養(yǎng)條件,網站上有細胞照片,聯系郵箱xiangfbio@163.com.詳情請登陸http://www.xiangbio.com/歡迎各位老師來電咨詢!
iec-6 大鼠小腸隱窩上皮細胞
馬-達二氏犬腎細胞系 MDCK (NBL-2) CRL-34
鴨子胚胎成纖維細胞 Duck embryo CCL-141
小鼠胚胎成纖維細胞 3T3-L1 CCL-173
人結腸直腸腺癌 COLO 320DM CCL-220
人結腸直腸腺癌 NCI-H508 CRL-253
人結腸直腸腺癌 COLO 320HSR CCL-220.1
人B淋巴細胞 Ramos（RA1） CRL-1596
人淋巴母細胞 T2(174×CEM.T2) CRL-1992
大鼠心肌細胞 H9C2(2-1) CRL-1446
CRL-2007 JB6 Cl 30-7b 小鼠表皮細胞
豬鼻甲黏膜成纖維細胞 PT-K75 CRL-2528
小鼠胰腺癌細胞 LTPA CRL-2389
大鼠胰腺癌細胞 DSL-6A/C1 CRL-2132
小鼠骨髓瘤B淋巴細胞 SP2/MIL-6 CRL-2016
大鼠腦間質細胞 DI TNC1 CRL-2005
小鼠肌原細胞 C2C12 CRL-1772
鵪鶉纖維肉瘤細胞 QT6 CRL-1708
大鼠回腸細胞 IEC-18 CRL-1589
非洲綠猴腎細胞 VERO C1008 [Vero 76, clone E6, Vero E6] CRL-1586
人成纖維細胞 Hs 738.St/Int CRL-7869
人正常前列腺上皮細胞 WPE-int CRL-2888
人正常前列腺成肌纖維細胞 WPMY-1 CRL-2854
人胰腺導管腺癌細胞 PL45 CRL-2558
人胰腺癌細胞 Panc 05.04 CRL-2557
人胰腺癌細胞 Panc 04.03 CRL-2555
人胰腺癌細胞 Panc 02.03 CRL-2553
人胰腺癌細胞 Panc 08.13 CRL-2551
人胰腺癌細胞 Panc 03.27 CRL-2549
人胰腺癌細胞 Panc 10.05 CRL-2547
人肝癌細胞 SNU-398 CRL-2233
人成神經瘤細胞-骨髓 SK-N-DZ CRL-2149
人胰腺癌細胞 HPAC CRL-2119
人胰腺癌細胞 HPAF-II CRL-1997
人黑色素瘤細胞 A375.S2 CRL-1872
人臍靜脈內皮細胞 HUV-EC-C CRL-1730
人黑素瘤細胞 WM-266-4 CRL-1676
人膀胱癌細胞 HT-1376 CRL-1472
人正常前列腺上皮細胞 RWPE-2 CRL-11610
人正常前列腺上皮細胞 RWPE-1 CRL-11609
雞胚胎成纖維細胞 UMNSAH/DF-1 CRL-12203
人乳腺癌細胞 MDA-MB-231 HTB-26
CRL-2007 JB6 Cl 30-7b 小鼠表皮細胞
小鼠巨噬細胞 RAW 264.7 TIB-71
人橫紋肌肉瘤細胞 A-204 HTB-82
人胰腺癌細胞 Capan-2 HTB-80
人胰腺癌細胞 Capan-1 HTB-79
人乳腺導管癌細胞 MDA-MB-435S HTB-129
人胃癌細胞 Hs 746T HTB-135
人肺腺癌細胞 NCI-H441 [H441] HTB-174
人骨髓瘤細胞 U266 TIB-196
人結腸癌細胞 NCI-H716 [H716] CCL-251
人多形性惡性膠質瘤 T98G [T98-G] CRL-1690
人神經母細胞瘤細胞 BE(2)-C CRL-2268
人腎透明細胞癌細胞 Caki-2 HTB-47
人皮膚成纖維細胞 Hs 865.Sk CRL-7601
CA46 人淋巴瘤細胞系
CRL-5826 NCI-H226 人肺鱗癌細胞系
Tca-8113 人舌鱗癌細胞
MA-891 小鼠乳腺癌高轉移細胞
U14 小鼠宮頸癌細胞
CRL-9518 L5178Y 小鼠淋巴瘤細胞
HTB-112 HEC-1-A 人子宮內膜腺癌細胞
HTB-113 HEC-1-B 人子宮內膜腺癌細胞
AN3 CA 人子宮內膜腺癌細胞
ROS1728 大鼠成骨細胞
CRL-6323 B16F1 小鼠黑色素瘤細胞
HTB-58 SK-MES-1 人肺鱗癌細胞
CCL-127 IMR-32 人神經母細胞瘤細胞
HTB-79 Capan-2 人胰腺癌細胞
BALL-1 人B淋巴細胞急性白血病細胞
CRL-2007 JB6 Cl 30-7b 小鼠表皮細胞
CRL-2922 Eayh926 人臍靜脈細胞融合細胞
WB-F344 大鼠肝干細胞
CRL-1715 TM4 正常小鼠睪丸細胞
CRL-2539 4T1 小鼠乳腺癌細胞
CRL-1442 BRL-3A 大鼠肝細胞
BRL 大鼠肝細胞
RSC96大鼠雪旺細胞
Isk 人子宮內膜癌細胞
HTB-113 hec-1-b 人子宮內膜腺癌細胞
PLA-802?人惡性橫紋肌肉瘤
Pt-K2 袋鼠腎細胞
BS-C-1 非洲綠猴腎細胞
A498人腎癌細胞
3AO人卵巢癌細胞
iec-6 大鼠小腸隱窩上皮細胞
u87MG 人腦星形膠質母細胞瘤
Renca 小鼠腎癌細胞
nci-h929 骨髓瘤細胞
P388小鼠白血病細胞
CRL-2007 JB6 Cl 30-7b 小鼠表皮細胞
opm-2 骨髓瘤細胞
MDA-MB-435 人乳腺癌高轉移細胞
KU812 人外周血嗜堿性白細胞
NIH:OVCAR-3 人卵巢癌細胞
SW 1990人胰腺癌細胞系
S180人結腸腺癌細胞
HuTu-80十二指腸腺癌
RL95-2 人子宮內腺癌細胞
NCI-H446 人小細胞肺癌細胞
MSTO-211H 人肺癌細胞
H4-Ⅱe 大鼠肝癌細胞系
HLF 人肺成纖維樣細胞
LS 180人結腸腺癌細胞
HuTu-80十一指腸腺癌
ST豬睪丸細胞系
REH人急性非B非T淋巴細胞白血病
NCI-H520 人肺鱗癌細胞
RKO 人結腸腺癌細胞
293A 人胚腎細胞
RK-13 兔腎細胞系
李(先生) （來電時請說是從給覽網看到我的)
免責聲明：以上所展示的[CRL-2007 JB6 Cl 30-7b 小鼠表皮細胞]信息由會員[上海復祥生物科技有限公司]自行提供，內容的真實性、準確性和合法性由發(fā)布會員負責。
