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價格：電議
所在地：上海
型號：SG CL-202
手機：15000266580
電話：400-821-8510
更新時間：2018-01-08
瀏覽次數：822
公司地址：上海市虹口區赤峰路630弄南雁大廈
15000266580 
李(先生) （來電時請說是從給覽網看到我的)
1.適用于過氧化物檢測的細胞發光增強劑
2.使用方便特別適用于過氧化物
3.增強信號100-600倍
4.只需要非常少的細胞且對細胞性
Diogenes細胞化學發光增強系統是一種過氧化物化學發光增強劑，不會使活細胞變性失活。超氧自由基會在細胞有氧呼吸或者白細胞抵御感染的過程中產生。白細胞在受到fMLP、佛波酯、抗Fc受體的抗體以及LPS刺激時進行氧化反應的程度可以部分作為免疫活力的指標。現在測定白細胞產生的超氧自由基的方法都非常的繁瑣而且不直接，例如在氰化物存在或缺失的條件下測定克拉克氏電對氧氣的吸收，或者測定細胞色素c被還原時發生的光譜改變。Diogene試劑盒利用生成性中間產物的發光反應，非常適用于細胞介導的過氧化物生成檢測。過氧化物氧化反應發射化學熒光的強弱與超氧自由基的濃度成正比，而且比使用魯米諾發出的光強很多。因此Diogenes非常適合于檢測細胞的免疫活性，只需要使用化學光度計便可定量光信號的強弱。任何刺激氧化酶產生胞外過氧化物的反應都可以用Diogenes檢測。可以在生理條件下或者模仿生理條件下完成過氧化物的檢測。
Diogenes Cellular Luminescence Enhancement System is a superoxide chemiluminescent enhancer that is non-denaturing to living cells. Superoxide radical (O2-) is produced intracellularly as a consequence of aerobic metabolism and extracellularly by leukocytes in response to infection. The extent of oxidative burst produced by white blood cells (WBCs) when stimulated by f-met-leu-phe, phorbol esters, anti-Fc receptor antibodies or LPS is a partial indicator of the immunocompetence of the cells tested.
Currently, the production of O2- by leukocytes is monitored by such cumbersome and indirect methods as measuring oxygen uptake in a Clark electrode (both in the presence and absence of cyanide) or measuring spectral changes caused by the reduction of cytochrome c. As a non-cytotoxic intermediate in the mechanism of photon production, Diogenes is ideally suited to the detection of cell-mediated superoxide production. The intensity of light produced by Diogenes in the presence of superoxide is directly proportional to the O2- concentration, but is much higher than that achieved by using luminol. Therefore, Diogenes is ideal for monitoring cellular immunocompetence, utilizing a luminometer to quantify the light output. Any stimulant that activates an oxidase to produce extracellular superoxide is usable with Diogenes. Such means can be physiological ormimetic of the physiologic pathway.
免責聲明：以上所展示的[SG CL-202 Diogenes 細胞化學發光增強試劑盒]信息由會員[上海復祥生物科技有限公司]自行提供，內容的真實性、準確性和合法性由發布會員負責。
