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價格：電議
所在地：上海
型號：SG CL-300
手機：15000266580
電話：400-821-8510
更新時間：2016-07-11
瀏覽次數(shù)：765
公司地址：上海市虹口區(qū)赤峰路630弄南雁大廈
15000266580 
李(先生) （來電時請說是從給覽網(wǎng)看到我的)
1.市場上的加強型Western Blot化學(xué)發(fā)光底物
2.超長發(fā)光時間（120分鐘)，以利于優(yōu)化性噪比
3.敏感性高，需要更少的抗體
4.保質(zhì)期長 (4攝氏度保存一年)
試劑盒采用的化學(xué)物質(zhì)可以將WB的敏感度提高20倍.從而可以檢測到豐度更低的目的蛋白或者使用更高稀釋度的一抗二抗,從而大大的節(jié)約成本.試劑盒可以在120分鐘內(nèi)保持穩(wěn)定的發(fā)光, 從而保證每次曝光每張膠片都可以得到穩(wěn)定的實驗結(jié)果.也可以從時間上允許多次曝光以選取合適的性噪比。可以檢測到5ng的目標(biāo)蛋白，將抗體稀釋度提高4到40倍仍然可以得到良好的實驗結(jié)果,從而大大節(jié)約昂貴的抗血清成本.
National Diagnostics' ProtoGlow ECL delivers the latest technology developed for enhances chemiluminescent detection on Western blots. The unique chemistry of the ProtoGlow ECL system increases the sensitivity of Western blots by up to 20 fold. This allows the detection of proteins at lower abundance and/or the use of higher dilutions of primary and secondary antibodies, economizing on these expensive reagents.
The improved sensitivity of ProtoGlow ECL is demonstrated below. Serial dilutions of transferrin were blocked and probed with HRP labelled antibody, and then subjected to detection using ProtoGlow ECL. ProtoGlow ECL detected down to 5ng of transferrin.
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| Gel electroblotted onto nitrocellulose and probed with HRP-labelled antibody at 1:30,000 dilution. Detection using ProtoGlow ECL. Blots were exposed for 90 seconds. Lane 1) 100 ng; Lane 2) 50 ng; Lane 3) 25 ng; Lane 4) 10 ng; Lane 5) 5 ng. |
ProtoGlow ECL generates a consistent light output for as long as 120 minutes. This ensures results are more reproducible: exposure to exposure, blot to blot. It also allows multiple exposures of a single blot to be taken to optimize the signal:noise ratio.
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| Sequential exposures of serially diluted HRP conjugated anti-transferrin antibody detected with ProtoGlow ECL. Exposures of 60 seconds were taken at 0, 30, 60 and 180 minutes. Dilutions: Lane 1) 1:10,000; Lane 2) 1:20,000; Lane 3) 1:40,000; Lane 4) 1:80,000; Lane 5) 1:160,000. |
ProtoGlow ECL allows the researcher to use less antibody. The extremely enhanced signal from ProtoGlow ECL allows you to use anywhere from 4 to 40 times less antibody per blot while retaining the same detection sensitivity. This in turn allows you to economize on the consumption of expensive antisera.
免責(zé)聲明：以上所展示的[SG CL-300 化學(xué)發(fā)光顯色（ECL）試劑盒]信息由會員[上海復(fù)祥生物科技有限公司]自行提供，內(nèi)容的真實性、準(zhǔn)確性和合法性由發(fā)布會員負責(zé)。
