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企業(yè)認(rèn)證：
價(jià)格：電議
所在地：北京
型號(hào)：BMEG02
更新時(shí)間：2024-09-23
瀏覽次數(shù)：1277
公司地址：北京市海淀區(qū)北太平莊路2號(hào)院2號(hào)QQ：1767180321
張鈺(女士) 業(yè)務(wù)經(jīng)理
hp-Vector
Features:
High performance vectors with optimized sequence
Protein yield up to 10 times enhanced compared to protein
Plasmids with established MCS
Encoding C- or N-terminal His-tag for versatile purification (native, 6xHis-tag)
Secretion with LipA or YocH signal peptide up to 9 fold increased
optimized high performance (hp) vectors for Bacillus megaterium!
MoBiTec provides a wide range of useful vectors for the B. megaterium system that are adaptable to most applications and protein purification methods. These include vectors carrying a secretion signal peptide sequence and a 6xHis-tag.
With our new hp-vectors yields up to 10-fold higher compared to the basic plasmids are possible. All plasmids have established multiple cloning sites (MCS) for versatile cloning. Furthermore we offer vectors encoding C- or N-terminal His-tags for easy purification. The protein secretion with LipA or YocH signal peptides is up to nine-fold increased. Induction of protein
Versatile system with a wide range of vectors
The B. megaterium
B. megaterium has proven to be an excellent alternative host to E. coli for heterologous gene
B. megaterium protoplasts specifically optimized for transformation
The protoplasts supplied by MoBiTec are from B. megaterium strain WH320, developed by Prof. Dr. W. Hillen at the Institute of Microbiology in Erlangen, Germany. These protoplasts are prepared according to an optimized protocol resulting in the highest transformation efficiencies.
For secretion vectors we offer protoplasts of strain MS941. Both strains are mutants of DSM319, where WH320 is a chemically mutant, while MS941 has a defined deletion in the gene of the major extracellular protease NprM.
Order Information, shipping and storage
Please click on the respective order # for further details.
order# de
BMEG02 Bacillus megaterium protoplasts ready for transformation (strain WH320;1)
Material is sufficient for 4 transformations plus control experiment. 5 x 500 µl
BMEG50 Bacillus megaterium protoplasts, strain MS941 5 x 500 µl
BMEG31 pC-His1623hp shuttle vector, C-term. 6xHis-tag; lyophilized DNA 10 μg
BMEG32 pN-His-TEV1623hp shuttle vector, N-term. His-tag,TEV-site 10 μg
BMEG30 p3S623hp, shuttle vector, lyophilized DNA 10 μg
BMEG33 pSPLipA-hp, shuttle vector with signal sequence, lypophilized DNA 10 μg
BMEG34 pSPYocH-hp, shuttle vector with signal sequence, lypophilized DNA 10 µg
shipped at RT, protoplasts shipped on dry ice
store lyophilized vectors at 4°C, reconstituted vectors at -20°C, protoplasts at -70°C
All vectors are E. coli / B. megaterium shuttle vectors
Please note that protoplasts of Bacillus megaterium have a limited shelf life of some months only, depending on the date of production. Therefore purchase and use of protoplasts must be thoroughly planned.
北京畢特博生物技術(shù)有限責(zé)任公司
：張鈺
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QQ：1767180321
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網(wǎng)站：
www.bitebo.com
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免責(zé)聲明：以上所展示的[BMEG02 hp-Vector表達(dá)系統(tǒng)megaterium芽孢桿菌]信息由會(huì)員[北京畢特博生物技術(shù)有限責(zé)任公司]自行提供，內(nèi)容的真實(shí)性、準(zhǔn)確性和合法性由發(fā)布會(huì)員負(fù)責(zé)。
