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26039 BRAF(V600E) 小鼠單抗/推薦商家

武漢費斯德生物科技有限公司

會員指數：

企業認證：

價格：電議

所在地：湖北武漢市

型號：26039

更新時間：2023-04-16

瀏覽次數：714

公司地址：武漢東湖新技術開發區高新大道666號武漢國家生物產業基地項目(生物創新園)B.C.D區研發樓B3-3樓301室

18162422647 付蘭蘭(先生)

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產品簡介

BRAF(V600E) 小鼠單抗/推薦商家品牌: 美國 NewEast Biosciences貨號: 26039抗原: Synthetic peptide規格: 100 微升產品全名: Anti-BRaf(V600E) Mouse Monoclonal Antibody應用: ELISA, WB, IF, IHC種屬反應性的選擇: Human, Mouse and Rat

公司簡介

武漢費斯德生物科技公司成立于2022年，坐落于生物產業基地——武漢光谷生物城，是一家集科研、生產、銷售為一體的專業化生物企業，致力于，為所有科研用戶提供高質量的生物產品，及一站式的科學技術服務。經營著抗體、蛋白、科研試劑盒等上百種類型產品。可滿足生物化學、分子生物學、細胞生物學、免疫學 ELISA試劑盒等生物科技實驗需求。我公司將向你提供以下的三種抗體或者試劑盒: (1) 僅識別 GTP酶的活性構型的產品, 它可以讓你能夠量化GTP酶在細胞中的活性和分布。(2) 識別突變 Oncogene蛋白, 但不認識相應野生型的抗體。 (3) 對 cAMP 和 cGMP 具有超親和力（無需乙酰化）ELISA檢測試劑盒。這些產品被將近一千篇同行評議的文章所引用，其中文章部分收錄在前50知名期刊。現客戶群體現已覆蓋大多科研院校、生物公司、科研企業等，我們同時也是美國NewEast Biosciences中國區域總代理商。 NewEast Biosciences 在十二年前研發倆種獨特的抗體，這倆種抗體僅僅識別活性的GTP酶或者突變的Oncogene。GTP酶涉及(1)響應細胞表面受體激活的信號轉導，包括跨膜受體，例如介導味覺、嗅覺和視覺的那些，（2）核糖體的蛋白質生物合成，（3）調節細胞分化、增殖、分裂和運動，（4）蛋白質通過膜的易位，（5）細胞內囊泡的運輸，以及囊泡介導的分泌和攝取，通過GTP酶控制囊泡外殼組裝，Oncogene側是誘發癌癥的基因。我們將在未來的工作中，以為人類生命科學研究提供好的科研產品為核心，給廣大客戶提供全方位的產品及服務。本公司銷售的所有產品僅供實驗科研使用，不用于人體及臨床診斷。

展開

產品說明

貨號: 26039

基因符號: BRaf

描述: Anti-BRaf(V600E) Mouse Monoclonal Antibody

背景介紹: B-Raf is a member of the Raf family of Ser/Thr protein kinases. It functions downstream of Ras to regulate the MAP kinase signaling pathway. Mutations in the BRAF gene cause diseases. Inherited mutations in BRAF cause cardiofaciocutaneous syndrome. Acquired mutations in BRAF have been found in cancers

免疫原: A synthetic peptide from the internal region of BRaf(V600E), human origin

經過測試的應用: ELISA, WB, IHC, IP

推薦稀釋比:
ELISA: 1: 100-1: 5000
WB: 1: 100-1: 2000
IHC: 1: 100-1: 1000

濃度: 1 mg/ml

宿主: Mouse

形式: Liquid

克隆性: Monoclonal

亞型: IgG2b

純度: Purified from ascites

防腐劑: No

成分: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol

種屬反應性: Recognizes B-Raf(V600E), but not wild type B-Raf proteins from vertebrates.

儲存條件: Store at -20°C. Avoid repeated freezing and thawing

蛋白質印跡:

Western blot analysis of recombinant B-raf proteins with anti-BRaf(V600E) antibody.

Purified His – tagged B-Raf( V600E ) ?amino acids 513-693)(lane1) ? corresponding wild type protein(lane 2), His – tagged B-Raf( V600D ) (lane 3) , His – tagged B-Raf( V600A ) (lane 4) , His – tagged B-Raf( V600G ) (lane 5) , His – tagged B-Raf( V600K ) (lane 6), His – tagged B-Raf( V600R ) (lane 7), and His – tagged B-Raf( V600M ) (lane 8) were blotted with anti-B-Raf(V600E) monoclonal antibody (Cat. # 26039) .

免疫熒光:

Immunofluorescence of cell expressing BRaf protein with anti-BRaf(V600E) antibody . HEK293T cells were transfected with pCDNA3-GFP- B-Raf( V600E )plasmid,pCDNA3-GFP- B-Raf( WT )plasmid, pCDNA3-GFP- B-Raf( V600K )plasmid,pCDNA3-GFP- B-Raf( V600R )plasmid, pCDNA3-GFP- B-Raf( V600A )plasmid,pCDNA3-GFP- B-Raf( V600D ) plasmid, pCDNA3-GFP-B-Raf(V600M)plasmid, or pCDNA3-GFP-B-Raf(V600G)plasmid, then fixed and stained with anti-B-Raf(V600E) monoclonal antibody (Cat. # 26039).

Immunohistochemistry:

B-Raf(V600E) monoclonal antibody (Cat. # 26039). Tissue samples were fixed with formaldehyde and blocked with 1% serum for 15 min at 37 °C. Antigen retrieval was by heat mediation in citrate buffer (pH6). Samples were then incubated with primary antibody (1: 100) overnight at 4°C. A HRP-conjugated Goat anti-mouse IgG (dilution 1: 50) was used as secondary antibody.

Immunohistochemical analysis of paraffin-embedded Thyroid Carcinoma tissue -with anti-BRaf (V600E) monoclonal antibody (Cat. # 26039). Tissue samples were fixed with paraffin. Samples were then incubated with primary antibody (1: 100) overnight at 4°C. An HRP-conjugated Goat anti-mouse IgG (dilution 1: 50) was used as the secondary antibody. Allele-specific PCR validated to be Negative for BRAF V600E.

圖片.png

Immunohistochemical analysis of paraffin-embedded Thyroid Carcinoma tissue -with anti BRaf(V600E) monoclonal antibody (Cat. # 26039). Tissue samples were fixed with paraffin. Samples were then incubated with primary antibody (1: 100) overnight at 4°C.A HRP-conjugated Goat anti-mouse IgG (dilution 1: 50) was used as secondary antibody.Allele specific PCR validated to be Negative for BRAF V600E.
BRAF(V600E) 小鼠單抗/推薦商家BRAF(V600E) 小鼠單抗/推薦商家BRAF(V600E) 小鼠單抗/推薦商家

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