| Catalogue Number |
CYT-326 |
| Synonyms |
Urogastrone, URG, EGF. |
| Introduction |
Epidermal growth factor has a profound effect on the differentiation of specific cells in vivo and is a potent mitogenic factor for a variety of cultured cells of both ectodermal and mesodermal origin. The EGF precursor is believed to exist as a membrane-bound molecule which is proteolytically cleaved to generate the 53-amino acid peptide hormone that stimulates cells to divide. EGF stimulates the growth of various epidermal and epithelial tissues in vivo and in vitro and of some fibroblasts in cell culture. |
| Description |
Epidermal Growth Factor Mouse Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 53 amino acids including 3 intramolecular disulfide-bonds and having a molecular mass of 6 kDa.
The EGF is purified by proprietary chromatographic techniques. |
| Source |
Escherichia Coli. |
| Physical Appearance |
Sterile Filtered White lyophilized (freeze-dried) powder. |
| Formulation |
The protein was lyophilized from a concentrated (1mg/ml) solution containing 1x PBS pH-7.4. |
| Solubility |
It is recommended to reconstitute the lyophilized Epidermal Growth Factor in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions. |
| Stability |
Lyophilized Epidermal Growth Factor Recombinant although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution EGF should be stored at 4°C between 2-7 days and for future use below -18°C.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please prevent freeze-thaw cycles. |
| Purity |
Greater than 97.0% as determined by(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE. |
| Amino acid sequence |
The sequence of the first five N-terminal amino acids was determined and was found to be Asn-Ser-Tyr-Pro-Gly, which agrees with the sequence of native Epidermal Growth Factor human. |
| Biological Activity |
The ED50, calculated by the dose-dependant proliferation of murine BALB/c 3T3 cells (measured by 3H-thymidine uptake) is < 0.1 ng/ml corresponding to a specific activity of 10MUnits/mg. |
| Protein content |
Protein quantitation was carried out by two independent methods
1. UV spectroscopy at 280 nm using the absorbency value of 3 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics).
2. Analysis by RP-HPLC, using a calibrated solution of EGF as a Reference Standard. |
| Usage |
ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals. |
| Related Products |
| » EGF (Leu 21) Human |
| » EGF Antibody |
| » EGF Human |
| » EGF Human, Pichia |
| » EGF Mouse |
| » EGF Rat |
| » EGF Rat |
|
2011年底 生化試劑-大促銷
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貨號(hào)CYT-209 重組人白介素-2 Human IL-2 10µg ￥900
貨號(hào)CYT-211重組人白介素-4 IL-4 2µg ￥900
貨號(hào)CYT-213 重組人白介素-6 IL-6 5µg ￥900
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貨號(hào)CYT-388 重組大鼠白介素-6 rIL-6 2µg ￥900
貨號(hào)CYT-394 重組大鼠白介素-1β rIL 1 beta 2µg ￥900
貨號(hào)CYT-405 重組豬腫瘤壞死因子-a rpTNF-a 5µg ￥900
貨號(hào)CYT-446A 重組人白介素-13 IL 13 2µg ￥900
貨號(hào)CYT-569 重組人活化素-A Activin-A 2µg ￥900
貨號(hào)CYT-569 重組人活化素-A Activin-A 2µg ￥900
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貨號(hào)ENZ-308 Taq DNA聚合酶（4支一套） TaqDNA 1,000U ￥900
貨號(hào)PRO-445 U1snRNP-68 U1snRNP-68 20µg ￥1,900
Human HDL 高密度脂蛋白膽固醇 Specifications ：MF, AF, C (10mg/ml)；Source：Human blood ；Purity：98% 2mg 1200
DiI-HDL DiI 熒光標(biāo)記高密度脂蛋白 pecifications ：MF, AF , C；Source：Human blood；Purity：98% ；300ug 2300
[I125] Human HDL 碘125標(biāo)記人高密度脂蛋白pecifications ：MF, AF, (100-300cpm/ng)； Source：Human blood； 300uCi 2800
Human LDL人低密度脂蛋白 pecifications ：MF, AF, C (2-4mg/ml；Source：Human blood；Purity ：98% 2mg 1200
DiI-LDL DiI標(biāo)記人低密度脂蛋白 pecifications ：MF, AF, C；Source ：Human blood 300ug 2300
[I125] Human LDL 碘125標(biāo)記人低密度脂蛋白 pecifications ：MF, AF, (100-300cpm/ng) , C；Source ：Human blood；Purity ：98% 100uCi 2600
DiO-LDL DiO標(biāo)記人低密度脂蛋白 pecifications ：MF, AF, C；Source ：Human blood 300ug 2700
Human Ac-LDL 人乙酰化低密度脂蛋白 pecifications ：MF, AF, (2mg/ml), C；Source ：Human blood；Purity ：98% 2mg 1200
DiI-Ac-LDL DiI標(biāo)記乙酰化人低密度脂蛋白 pecifications ：MF, AF, (200ug/ml), C；Source ：Human blood 200ug 2800
Dio-Ac-LDL DiO標(biāo)記乙酰化人低密度脂蛋白 pecifications ：MF, AF, (200ug/ml), C；Source ：Human blood 300ug 2300
[I125] Human Ac-LDL 碘125標(biāo)記乙酰化人低密度脂蛋白 pecifications ：MF, AF, (100-300cpm/ng), C；Source ：Human blood；Purity ：98% 100uCi 2600
Human Ox-LDL 人氧化低密度脂蛋白 pecifications ：MF, AF, Tbars, (1.5-2.5mg/ml), C；Source ：Human blood；Purity ：98% 2mg 1200
DiI-Ox-LDL DiI標(biāo)記人氧化低密度脂蛋白 pecifications ：MF, AF, (200ug/Vial), C；Source ：Human blood 300ug 2300
[I125] Ox-LDL 碘125標(biāo)記人氧化低密度脂蛋白 pecifications ：MF, AF, Tbars, (100-300cpm/ng), C ；Source ：Human blood；Purity ：98% 100uCi 2600
Human Lp[a] 人脂蛋白a pecifications ：Contains 0.01% Azide ；Source ：Human blood；Purity ：98% 2mg 1200
Human VLDL 人極低密度脂蛋白 pecifications ：MF, AF, C (1-2mg/ml) ；Source ：Human blood；Purity ：98% 2mg 1200
DiI-VLDL DiI標(biāo)記的人極低密度脂蛋白
[I125] Human VLDL 碘125標(biāo)記的人極低密度脂蛋白
Lipoprotein Deficient H.Plasma 去脂蛋白血清
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