產(chǎn)品簡(jiǎn)介
Catalogue Number CYT-211 Synonyms BCGF, BCDF,
公司簡(jiǎn)介
上海亞培生物科技有限公司，是一家專注于生命科學(xué)和生物技術(shù)領(lǐng)域的高科技企業(yè)，專業(yè)提供分子生物學(xué)、免疫學(xué)、生命科學(xué)基礎(chǔ)研究以及臨床檢測(cè)等諸多領(lǐng)域的試劑、耗材、儀器等各類產(chǎn)品及生物技術(shù)服務(wù)。 公司一方面注意跟蹤世界范圍分子生物學(xué)、細(xì)胞生物學(xué)等生物學(xué)科的發(fā)展前沿，努力將歐美專業(yè)生產(chǎn)廠家的具有國(guó)際先進(jìn)水平的產(chǎn)品推薦給各類研究人員；另一方面也非常重視自主產(chǎn)品研究和開發(fā)，推出了一系列具有競(jìng)爭(zhēng)力的產(chǎn)品和服務(wù)。公司客戶遍布大學(xué)、研究所、醫(yī)院、衛(wèi)生防疫、商品檢驗(yàn)檢疫、制藥公司、生物技術(shù)公司和食品工業(yè)等單位。 公司在重視產(chǎn)品質(zhì)量的同時(shí)，也建立了一套集技術(shù)支持、物流、售后服務(wù)等多個(gè)部門的全方位的服務(wù)體系，努力把我們方便、快捷、周到的服務(wù)提供給每一個(gè)客戶。我們的目標(biāo)是讓亞培成為在生命科學(xué)和生物技術(shù)領(lǐng)域內(nèi)集生產(chǎn)、研究開發(fā)和銷售于一體的國(guó)內(nèi)外知名的一流企業(yè)每一個(gè)亞培人，都心存一個(gè)信念：希望通過自身的不懈努力，通過我們優(yōu)秀的產(chǎn)品、專業(yè)的服務(wù)來更好的服務(wù)用戶，為我們每一個(gè)用戶的成功鋪路搭橋，也為亞培的成功鋪路搭橋！
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產(chǎn)品說明
| Catalogue Number |
CYT-211 |
| Synonyms |
BCGF, BCDF, B cell stimulating factor, BSF-1, Lymphocyte stimulatory factor 1, IL-4, MGC79402, Binetrakin, Pitrakinra. |
| Introductionn |
IL4 is a pleiotropic cytokine produced by activated T cells. IL4 is a ligand for interleukin 4 receptor. The interleukin 4 receptor also binds to IL13, which may contribute to many overlapping functions of this cytokine and IL13. STAT6, a signal transducer and activator of transcription, has been shown to play a central role in mediating the immune regulatory signal of this cytokine. This gene, IL3, IL5, IL13, and CSF2 form a cytokine gene cluster on chromosome 5q, with this gene particularly close to IL13. IL4, IL13 and IL5 are found to be regulated coordinately by several long-range regulatory elements in an over 120 kilobase range on the chromosome. Two alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported. |
| Description |
Interleukin-4 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 130 amino acids and having a molecular mass of 15000 Dalton.
The IL-4 is purified by proprietary chromatographic techniques. |
| Source |
Escherichia Coli. |
| Physical Appearance |
Sterile Filtered White lyophilized (freeze-dried) powder. |
| Formulation |
Lyophilized from a concentrated (1mg/ml) solution in water containing no additives. |
| Solubility |
It is recommended to reconstitute the lyophilized Interleukin-4 in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions. |
| Stability |
Lyophilized Interleukin-4 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution IL4 should be stored at 4°C between 2-7 days and for future use below -18°C.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please prevent freeze-thaw cycles. |
| Purity |
Greater than 98.0% as determined by(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE. |
| Amino acid sequence |
The sequence of the first five N-terminal amino acids was determined and was found to be Met-His-Lys-Cys-Asp. |
| Biological Activity |
The ED50 as determined by the dose-dependant stimulation of TF-1 cells is < 0.2 ng/ml, corresponding to a Specific Activity of 5,000,000 IU/mg. |
| Protein content |
Protein quantitation was carried out by two independent methods
1. UV spectroscopy at 280 nm using the absorbency value of 0.594 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics).
2. Analysis by RP-HPLC, using a standard solution of IL-4 as a Reference Standard. |
| Usage |
ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals. |
| References |
|
| Related Products |
| » IL 4 Antibody |
| » IL 4 Antibody |
| » IL 4 Human, CHO |
| » IL 4 Human, His |
| » IL 4 Mouse |
| » IL 4 Porcine |
| » IL 4 Rat |
|
火熱促銷中....
Citric acid monohydrate Buffer grade 無水檸檬酸 1kg
Xanthosine dihydrate BioChemica 黃嘌呤核苷 1g
Taq DNA Polymerase Reaction buffer (10X) complete Taq DNA聚合酶 反應(yīng)緩沖液 5ml
Chloramphenicol pure Ph. Eur. 氯霉素 50g
D-Glucosamine hydrochloride BioChemica D-葡萄糖胺 250g
tri-Sodium citrate dihydrate Molecular biology grade 二水檸檬酸鈉 100g
Dextran 40000 BioChemica 葡聚糖 4000 25g
Ampicillin sodium salt pure Ph. Eur. 氨芐青霉素鈉鹽 25g
Carbenicillin disodium salt BioChemica 羥芐青霉素二鈉 1g
HEPES sodium salt Buffer grade HEPES鈉鹽 50g
Ampicillin sodium salt BioChemica 氨芐青霉素鈉鹽 10g
EGTA Molecular biology grade 乙二醇雙(2-氨基乙基)四乙酸 5g
Tris : Tris • HCl - Mixture (pH 8.8) Tris :HCl (pH 8.8) 100g
EDTA disodium salt dihydrate 乙二胺四乙酸二鈉/EDTA二鈉 250g
L-Phenylalanine pure Ph. Eur., USP L-苯丙氨酸 100g
Azure B (C.I. 52010) 天青B/天藍(lán)B /三甲硫紫 5g
Gentamycin sulfate BioChemica 慶大霉素硫酸鹽 1g
Kanamycin sulfate BioChemica 硫酸卡那霉素 5g
β-Cyclodextrin BioChemica β-環(huán)糊精 25g
Taq DNA Polymerase Reaction buffer (10X) complete Taq DNA聚合酶 反應(yīng)緩沖液 5ml
Polymyxin B sulfate BioChemica 多粘菌素B硫酸鹽 1g
Bicine Buffer grade N-二甘氨酸/N-二羥乙基甘氨酸 100g
Pyronin Y (C.I.45005) 派洛寧Y 5g
CHES Buffer grade CHES緩沖液 25g
MES monohydrate Molecular Biology grade MES(一水) 50g
Dimethyl sulfoxide pure Ph. Eur. 二甲基亞砜 250ml
Sodium citrate dihydrate p. A. 二水檸檬酸鈉 500g
TBS (Tris-buffered saline, 10X) TBS 500ml
Dextran 70000 BioChemica 葡聚糖 7000 25g
Adenine BioChemica 腺嘌呤 10g
Streptomycin sulfate pure Ph. Eur. 硫酸鏈霉素 100g
Thymine 胸腺嘧啶 5g
Xanthosine dihydrate BioChemica 黃嘌呤核苷 1g
Taq DNA Polymerase Reaction buffer (10X) complete Taq DNA聚合酶 反應(yīng)緩沖液 5ml
β-Cyclodextrin BioChemica β-環(huán)糊精 25g
Tris Buffer grade Tris緩沖液 500g
Tris-Tricine-SDS buffer (5X) BioChemica Tris-Tricine-SDS buffer (5X) 500ml
β-Cyclodextrin BioChemica β-環(huán)糊精 25g
Pyronin Y (C.I.45005) 派洛寧Y 5g
Xylene cyanol FF (C.I. 42135) BioChemica 二甲苯胺 10g
Bromocresol green 溴甲酚綠 1g
5-Fluorouracil (BioChemica) 5-氟尿嘧啶 5g
Safranine O (C.I. 50240) for Microscopy 番紅O/沙黃O 10g
Neutral red (C.I.50040) 中性紅 10g
Azure B (C.I. 52010) 天青B/天藍(lán)B /三甲硫紫 5g
Kanamycin sulfate BioChemica 硫酸卡那霉素 5g
ACES Buffer grade ACES緩沖液 25g
Polyethylene glycol 8000 BioChemica (PEG8000) PEG8000 500g
Choline chloride BioChemica 氯化膽堿 500g
Agarose medium EEO 瓊脂糖 中EEO 50g
Brilliant green (C.I. 42040) 亮綠/艷綠 25g
Neomycin sulfate pure Ph. Eur. 新霉素 25g
Ampicillin sodium salt pure Ph. Eur. 氨芐青霉素鈉鹽 25g
MOPSO Buffer grade MOPSO緩沖液 25g
Agarose high EEO 瓊脂糖 高EEO 50g
Tris Buffer grade Tris緩沖液 500g
Tween® 80 BioChemica 吐溫80 500ml
Rhodamin B (C.I. 45170) 羅丹明B 25g
Fast Green FCF (C.I. 42053) 快綠 25g
Dimethyl sulfoxide pure Ph. Eur. 二甲基亞砜 250ml
Citric acid monohydrate Buffer grade 無水檸檬酸 1kg
Rhodamin B (C.I. 45170) 羅丹明B 25g
Rhodamine 6G (C.I. 45160) 羅丹明6G 25g
Salicylic acid pure Ph. Eur., USP 水楊酸 500g
Sodium citrate dihydrate p. A. 二水檸檬酸鈉 500g
Fuchsin basic (C.I. 42510) Microscopy grade 品紅/洋紅 堿性 25g
Crystal violet (C.I. 42555) 結(jié)晶紫 25g
Rhodamin B (C.I. 45170) 羅丹明B 25g
Rhodamine 6G (C.I. 45160) 羅丹明6G 25g
Brij® 35 BioChemica Brij® 35 500g
Tween® 80 BioChemica 吐溫80 500ml
tri-Sodium citrate dihydrate Molecular biology grade 二水檸檬酸鈉 100g
Gentamycin sulfate BioChemica 慶大霉素硫酸鹽 1g
Agarose Low Melt Large DNA grade 瓊脂糖 低熔點(diǎn)（大DNA片段） 10g
L-Arginine base pure Ph. Eur., USP L-精氨酸 100g
MES monohydrate Molecular Biology grade MES(一水) 50g
MOPS Molecular biology grade MOPS 100g
CHES Buffer grade CHES緩沖液 25g
SDS pure Ph. Eur 十二烷基磺酸鈉 250g
Sodium citrate dihydrate p. A. 二水檸檬酸鈉 500g
Tetracycline hydrochloride BioChemica 四環(huán)素鹽酸鹽 25g
Neutral red (C.I.50040) 中性紅 10g
Safranine O (C.I. 50240) for Microscopy 番紅O/沙黃O 10g
5-Fluorouracil (BioChemica) 5-氟尿嘧啶 5g
Crystal violet (C.I. 42555) 結(jié)晶紫 25g
Polyethylene glycol 8000 BioChemica (PEG8000) PEG8000 500g
Tryptone BioChemica 胰蛋白胨 250g
Tricine BioChemica 三(羥甲基)甲基甘氨酸 100g
Betaine hydrochloride pure 甜菜堿 250g
Adenine BioChemica 腺嘌呤 10g
Chloramphenicol pure Ph. Eur. 氯霉素 50g
Tris Buffer grade Tris緩沖液 500g
SDS pure Ph. Eur 十二烷基磺酸鈉 250g
Neomycin sulfate pure Ph. Eur. 新霉素 25g
Dextran 70000 BioChemica 葡聚糖 7000 25g
Bromocresol green 溴甲酚綠 1g
Thymine 胸腺嘧啶 5g
Trypsin inhibitor from soybean >7000 BAEE 胰蛋白酶抑制劑 250mg
X-Gal Molecular Biology grade X-Gal 100mg
Trypsin inhibitor from soybean >10000 BAEE 胰蛋白酶抑制劑 250mg
Trypsinogen 胰蛋白酶原 250mg
Peroxidase from horseradish EIA and Immunology Grade I 過氧化物酶 1KU
RNase A RNA酶A 100mg
Chymostatin 抑凝乳蛋白酶素/抑糜蛋白酶素 5mg
α-Chymotrypsin from bovine pancreas Grade II 胰凝乳蛋白酶/糜蛋白酶 II級(jí) 500mg
Proteinase K - Solution 蛋白酶K溶液 1ml
Genistein BioChemica 染料木黃酮/染料木堿/染料木素 25mg
Hygromycin B - Solution 潮霉素B溶液 1ml
Bilirubin pure 膽紅素 1g
Lysozyme BioChemica 溶菌酶 1g
Peroxidase from horseradish EIA and Immunology Grade I 過氧化物酶 1KU
Protein Marker I (14-116) 蛋白質(zhì)marker I 14-116kD 500μl
Lipase from porcine pancreas 脂肪酶 （豬） 25g
Trypsin inhibitor from soybean >7000 BAEE 胰蛋白酶抑制劑 1g
DNA Marker Phage Lambda - Sty I DNA Marker （入噬菌體 酶切） 50μg
DNA Marker Phage Lambda - BstE II (lyophilised) DNA Marker （入噬菌體 酶切） 100μg
Glucose Oxidase from Aspergillus niger, low catalase 葡萄糖氧化酶 1ml
Antifade Antifade 0,5ml
DNA Marker Phage Lambda - BstE II DNA Marker （入噬菌體 酶切） 50μg
X-Gal Molecular Biology grade X-Gal 100mg
DNA pUC19 Plasmid pUC19質(zhì)粒DNA 0,05mg
Proteinase K - Solution 蛋白酶K溶液 1ml
DNA Phage T7 T7噬菌體DNA 0.05mg
DNA Marker Phage Lambda Hind III (lyophilised) DNA Marker （入噬菌體 酶切） 100μg
Glucose Oxidase from Aspergillus niger, low catalase 葡萄糖氧化酶 1ml
Taq DNA Polymerase DNA-free Taq DNA聚合酶 100U/ml
Antifade Antifade 0,5ml
Coenzyme Q10 BioChemica 輔酶Q /泛醌 10mg
X-Gal Molecular Biology grade X-Gal 100mg
DNA Marker Phage Lambda - Hind III DNA Marker （入噬菌體 酶切） 50μg
L-Glutathione reduced BioChemica L-谷胱甘肽 還原型 5g
Albumin, human 白蛋白 （人） 100mg
Amphotericin B BioChemica 兩性霉素B 50mg
Pepsin 胃蛋白酶 25g
Benzamidine hydrochloride BioChemica 鹽酸芐脒/鹽酸苯甲脒 10g
L-Glutathione oxidized BioChemica L-谷胱甘肽 氧化型 1g
Trypsin inhibitor from soybean >7000 BAEE 胰蛋白酶抑制劑 250mg
L-Glutathione oxidized BioChemica L-谷胱甘肽 氧化型 1g
TEMED 四甲基乙二胺 25ml
Lithium 3,5-diiodosalicylate 3,5-二碘水楊酸鋰 1g
β-Mercaptoethanol BioChemica β-巰基乙醇 100ml
Trypsin 1:250 from porcine pancreas 豬胰蛋白酶（1:250） 25g
Pepsinogen II, human 胃蛋白酶原II，人源 25μg
DL-α-Lipoic acid BioChemica DL-α-硫辛酸 5g
DTT Molecular biology grade 二硫蘇糖醇/DTT 5g
Amphotericin B BioChemica 兩性霉素B 50mg
Pepsin 胃蛋白酶 25g
CHAPS Molecular biology grade CHAPS 5g
DL-α-Lipoic acid BioChemica DL-α-硫辛酸 5g
S-MEM_ modified for spinner culture S-MEM 1L
Albumin_ human 白蛋白 （人） 100mg
β-Mercaptoethanol BioChemica β-巰基乙醇 100ml
Flavinadenindinukleotid - Dinatriumsalz - Dihydrat BioChemica 黃素腺嘌呤二核苷酸 100mg
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Pepsin 胃蛋白酶 100g
β-Mercaptoethanol BioChemica β-巰基乙醇 100ml
Bradford - Solution for Protein determination Bradford蛋白定量溶液 500ml
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rIGF-1 重組大鼠胰島素樣生長(zhǎng)因子-1 10μg
hTNF-a 重組人腫瘤壞死因子-a 10µg
Activin-A 重組人活化素-A 2µg
TaqDNA Taq DNA聚合酶（4支一套） 1,000U
Visfatin Hu 重組人內(nèi)脂素 25µg
mEGF 重組小鼠表皮生長(zhǎng)因子 100µg
Visfatin Hu 重組人內(nèi)脂素 5µg
rLeptin 重組大鼠瘦素 20µg
Human IGF-1 重組人胰島素樣生長(zhǎng)因子-1 20µg
rIL-6 重組大鼠白介素-6 2µg
mVEGF 重組小鼠血管內(nèi)皮生長(zhǎng)因子 2µg
IL-6 重組人白介素-6 5µg
PEDF 重組人色素上皮衍生因子 20µg
Human IL-2 重組人白介素-2 10µg
Activin-A 重組人活化素-A 2µg
IL-4 重組人白介素-4 2µg
EPO-a 重組人紅細(xì)胞生成素-a 5µg
rIL-2 重組大鼠白介素-2 5µg
IL 13 重組人白介素-13 2µg
VEGF 重組人血管內(nèi)皮生長(zhǎng)因子 2μg
mGMCSF 重組小鼠粒細(xì)胞巨噬細(xì)胞集落刺激因子 2μg
rIL 1 beta 重組大鼠白介素-1β 2µg
rpTNF-a 重組豬腫瘤壞死因子-a 5µg
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