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價格：電議
所在地：上海
型號：貨號CYT-222
更新時間：2012-11-04
瀏覽次數：875
公司地址：上海市蓮花南路1108弄
林小姐(女士)
上海亞培生物科技有限公司，是一家專注于生命科學和生物技術領域的高科技企業，專業提供分子生物學、免疫學、生命科學基礎研究以及臨床檢測等諸多領域的試劑、耗材、儀器等各類產品及生物技術服務。 公司一方面注意跟蹤世界范圍分子生物學、細胞生物學等生物學科的發展前沿，努力將歐美專業生產廠家的具有國際先進水平的產品推薦給各類研究人員；另一方面也非常重視自主產品研究和開發，推出了一系列具有競爭力的產品和服務。公司客戶遍布大學、研究所、醫院、衛生防疫、商品檢驗檢疫、制藥公司、生物技術公司和食品工業等單位。 公司在重視產品質量的同時，也建立了一套集技術支持、物流、售后服務等多個部門的全方位的服務體系，努力把我們方便、快捷、周到的服務提供給每一個客戶。我們的目標是讓亞培成為在生命科學和生物技術領域內集生產、研究開發和銷售于一體的國內外知名的一流企業每一個亞培人，都心存一個信念：希望通過自身的不懈努力，通過我們優秀的產品、專業的服務來更好的服務用戶，為我們每一個用戶的成功鋪路搭橋，也為亞培的成功鋪路搭橋！
| Catalogue Number | CYT-222 | |||||||||
| Synonyms | CSF-2, MGI-1GM, GM-CSF, Pluripoietin-alpha, Molgramostin, Sargramostim. | |||||||||
| Introduction | GMCSF is a cytokine that controls the production, differentiation, and function of granulocytes and macrophages. The active form of the protein is found extracellularly as a homodimer. This gene has been localized to a cluster of related genes at chromosome region 5q31, which is known to be associated with interstitial deletions in the 5q- syndrome and acute myelogenous leukemia. Other genes in the cluster include those encoding interleukins 4, 5, and 13. GM-CSF stimulates the growth and differentiation of hematopoietic precursor cells from various lineages, including granulocytes, macrophages, eosinophils and erythrocytes. |
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| De |
Granulocyte Macrophage Colony Stimulating Factor Mouse Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 125 amino acids and having a molecular mass of 14285.35 Dalton. GM-CSF Mouse is purified by proprietary chromatographic techniques. |
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| Source | Escherichia Coli. | |||||||||
| Physical Appearance | Sterile Filtered White lyophilized (freeze-dried) powder. | |||||||||
| Formulation | GM-CSF Mouse was lyophilized with no additives. | |||||||||
| Solubility | It is recommended to reconstitute the lyophilized Granulocyte Macrophage Colony Stimulating Factor in sterile 20mM AcOH (acetic Acid) not less than 100µg/ml, which can then be further diluted to other aqueous solutions. | |||||||||
| Stability | Lyophilized Granulocyte Macrophage Colony Stimulating Factor although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution GM-CSF should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles. |
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| Purity | Greater than 98.0% as determined by(a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE. |
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| Amino acid sequence | The sequence of the first five N-terminal amino acids was determined and was found to be Met-Ala-Pro-Thr-Arg. | |||||||||
| Biological Activity | The ED50 as determined by the dose-dependant stimulation of the proliferation of murine FDC-P1 cell line is < 0.2 ng/ml, corresponding to a Specific Activity of 5,000,000 IU/mg. | |||||||||
| Protein content | GM-CSF quantitation was carried out by two independent methods 1. UV spectroscopy at 280 nm using the absorbency value of 0.765 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics). 2. Analysis by RP-HPLC, using a calibrated solution of GM-CSF as a Reference Standard. |
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| References | 1. Title:Multigene/multisubtype HIV-1 vaccine induces potent cellular and humoral immune responses by needle-free intradermal delivery. Publication: Mol Ther. 2005 Dec;12(6):1197-205. Epub 2005 Aug 22. PMID: 16112909 Applications: The Mouse GM-CSF used for 2 purposes: 1. As an adjuvant for DNA vaccine which included seven plasmids encoding nine HIV-1 proteins. The mice were injected with the DNA vaccine together with recombinant mouse GM-CSF. 2. Used in Elisa. 2. Title: Neospora caninum: cloning and Publication: Exp Parasitol. 2010 Aug;125(4):357-62. Epub 2010 Mar 6. PMID: 20211619 Applications: Used for immunoblotting 3. Title: Intranasal Granulocyte-Macrophage Colony-Stimulating Factor Reduces the Aspergillus Burden in an Immunosuppressed Murine Model of Pulmonary Aspergillosis. Publication: Antimicrob Agents Chemother. 2008 February; 52(2): 716–718. Published online 2007 November 5 Applications: GM-CSF as tested a therapeutic potential in a murine model of pulmonary aspergillosis. In summary, this pilot study indicates that GM-CSF administered intranasally may be a novel therapeutic approach for the prevention or treatment of pulmonary fungal infections and may augment the efficacies of antifungal agents. GM-CSF was given intranasal. 4. Title: Dendritic Cell-Based Therapeutic Vaccination against Myeloma: Vaccine Formulation Determines Efficacy against Light Chain Myeloma Publication: The Journal of Immunology February 1, 2009 vol. 182 no. 3 1667-1673 http://www.jimmunol.org/content/182/3/1667.full |
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| Usage | ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals. | |||||||||
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免責聲明：以上所展示的[貨號CYT-222 小鼠粒細胞巨噬細胞集落刺激因子]信息由會員[上海亞培生物科技有限公司]自行提供，內容的真實性、準確性和合法性由發布會員負責。
